We present motion-free polarization-sensitive lensless microscopy capable of generating high-resolution birefringence images over a large field-of-view (FoV). The lensless microscopy technique has been proposed to enable high-resolution imaging over a large FoV with a cost-effective design. The typical design of lensless microscopes employs a 3-dimensional motorized stage for a sensor due to its working principle based on subpixel super-resolution technique and multi-height phase recovery algorithm. Moreover, conventional lens-free polarized microscope uses two sets of raw holograms acquired by rotating the image sensor. However, this approach requires excessive mechanical movement of the image sensor, which necessitates precise image alignment, limits the FoV induced by rotating the image sensor, and restricts the image acquisition time.
Here, we demonstrate the motion-free, high-resolution, large-area birefringence imaging capability of polarization-sensitive lensless microscopy. By changing the polarizing state, RGB color channel, and position of the illumination light using an LED matrix, the birefringence image can be reconstructed without any mechanical movements of the sample or sensor. Our proposed design of a lensless microscope is anticipated to enhance the applicability of portable microscopic imaging systems in medical diagnosis.